Detailed information of TA system TA134638

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54799..55053
• Replicon: plasmid unnamed1
• Accession: NZ_CP041920
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FPV29_RS23345
• Protein ID: WP_001351576.1
• Coordinates: 54799..55005 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54992..55053 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS23300	50351..50752	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
FPV29_RS23305	50685..50942	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
FPV29_RS23310	51035..51688	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
FPV29_RS24935	51786..51926	-	141	WP_001333237.1	hypothetical protein	-
FPV29_RS23320	52627..53484	-	858	WP_113446638.1	incFII family plasmid replication initiator RepA	-
FPV29_RS23325	53477..53959	-	483	WP_001273588.1	hypothetical protein	-
FPV29_RS23330	53952..54026	-	75	WP_001442103.1	RepA leader peptide Tap	-
FPV29_RS23340	54258..54515	-	258	WP_000083845.1	replication regulatory protein RepA	-
FPV29_RS23345	54799..55005	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54992..55053	+	62	NuclAT_1	-	Antitoxin
-	54992..55053	+	62	NuclAT_1	-	Antitoxin
-	54992..55053	+	62	NuclAT_1	-	Antitoxin
-	54992..55053	+	62	NuclAT_1	-	Antitoxin
FPV29_RS24940	55309..55383	-	75	Protein_61	endonuclease	-
FPV29_RS23355	55629..55841	-	213	WP_001299730.1	ANR family transcriptional regulator	-
FPV29_RS23360	55977..56537	-	561	WP_000139315.1	fertility inhibition protein FinO	-
FPV29_RS23365	56640..57500	-	861	WP_000704514.1	alpha/beta hydrolase	-
FPV29_RS23370	57559..58305	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / aadA5 / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..122471	122471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T134638 WP_001351576.1 NZ_CP041920:c55005-54799 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T134638 NZ_CP041920:c55005-54799 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT134638 NZ_CP041920:54992-55053 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin