Detailed information of TA system TA134637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4547276..4547878
• Replicon: chromosome
• Accession: NZ_CP041919
• Organism: Escherichia coli strain Ec40743

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FPV29_RS21995
• Protein ID: WP_000897305.1
• Coordinates: 4547567..4547878 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FPV29_RS21990
• Protein ID: WP_000356397.1
• Coordinates: 4547276..4547566 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPV29_RS21965	4543201..4544103	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FPV29_RS21970	4544100..4544735	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FPV29_RS21975	4544732..4545661	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FPV29_RS21980	4545991..4546233	-	243	WP_001087409.1	hypothetical protein	-
FPV29_RS21985	4546453..4546671	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
FPV29_RS21990	4547276..4547566	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FPV29_RS21995	4547567..4547878	-	312	WP_000897305.1	hypothetical protein	Toxin
FPV29_RS22000	4548107..4549015	+	909	WP_001162704.1	alpha/beta hydrolase	-
FPV29_RS22005	4549079..4550020	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FPV29_RS22010	4550065..4550502	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FPV29_RS22015	4550499..4551371	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FPV29_RS22020	4551365..4551964	-	600	WP_001295269.1	glucose-1-phosphatase	-
FPV29_RS22025	4552063..4552848	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T134637 WP_000897305.1 NZ_CP041919:c4547878-4547567 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T134637 NZ_CP041919:c4547878-4547567 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT134637 WP_000356397.1 NZ_CP041919:c4547566-4547276 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT134637 NZ_CP041919:c4547566-4547276 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7