Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3528419..3529089 Replicon chromosome
Accession NZ_CP041864
Organism Mycobacterium tuberculosis strain 1-0044P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ42_RS16565 Protein ID WP_003899954.1
Coordinates 3528419..3528763 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ42_RS16570 Protein ID WP_003899955.1
Coordinates 3528760..3529089 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ42_RS16535 3523491..3524351 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ42_RS16540 3524326..3524841 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ42_RS16545 3525081..3525374 + 294 WP_003416635.1 hypothetical protein -
FPJ42_RS16550 3525663..3526952 + 1290 WP_003416640.1 ATP-binding protein -
FPJ42_RS16555 3527299..3527733 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ42_RS16560 3527736..3528188 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ42_RS16565 3528419..3528763 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ42_RS16570 3528760..3529089 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ42_RS16575 3529627..3529974 + 348 WP_031654674.1 DUF2384 domain-containing protein -
FPJ42_RS16580 3529971..3530591 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FPJ42_RS16585 3530751..3532016 - 1266 WP_003912135.1 hypothetical protein -
FPJ42_RS16590 3532202..3532394 + 193 Protein_3275 hypothetical protein -
FPJ42_RS16595 3532641..3533675 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T133892 WP_003899954.1 NZ_CP041864:3528419-3528763 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T133892 NZ_CP041864:3528419-3528763 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT133892 WP_003899955.1 NZ_CP041864:3528760-3529089 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT133892 NZ_CP041864:3528760-3529089 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References