Detailed information of TA system TA133772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3572389..3573059
• Replicon: chromosome
• Accession: NZ_CP041862
• Organism: Mycobacterium tuberculosis strain 1-0047P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPJ44_RS16725
• Protein ID: WP_003899954.1
• Coordinates: 3572389..3572733 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPJ44_RS16730
• Protein ID: WP_003899955.1
• Coordinates: 3572730..3573059 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ44_RS16700	3568423..3568812	+	390	Protein_3297	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPJ44_RS16705	3569052..3569345	+	294	WP_003416635.1	hypothetical protein	-
FPJ44_RS16710	3569633..3570922	+	1290	WP_003416640.1	ATP-binding protein	-
FPJ44_RS16715	3571269..3571703	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPJ44_RS16720	3571706..3572158	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ44_RS16725	3572389..3572733	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ44_RS16730	3572730..3573059	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPJ44_RS16735	3573597..3573944	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPJ44_RS16740	3573941..3574561	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPJ44_RS16745	3574721..3575986	-	1266	WP_003902423.1	hypothetical protein	-
FPJ44_RS16750	3576154..3576363	+	210	WP_003416778.1	hypothetical protein	-
FPJ44_RS16755	3576398..3576583	+	186	WP_015630737.1	hypothetical protein	-
FPJ44_RS16760	3576610..3577644	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T133772 WP_003899954.1 NZ_CP041862:3572389-3572733 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T133772 NZ_CP041862:3572389-3572733 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT133772 WP_003899955.1 NZ_CP041862:3572730-3573059 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT133772 NZ_CP041862:3572730-3573059 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6