Detailed information of TA system TA133635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2262144..2263063
• Replicon: chromosome
• Accession: NZ_CP041860
• Organism: Mycobacterium tuberculosis strain 1-0056P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: FPJ46_RS10530
• Protein ID: WP_031751732.1
• Coordinates: 2262458..2263063 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ46_RS10525
• Protein ID: WP_003410124.1
• Coordinates: 2262144..2262449 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ46_RS10490	2257407..2257607	+	201	Protein_2073	hypothetical protein	-
FPJ46_RS10500	2258963..2259340	+	378	Protein_2075	hypothetical protein	-
FPJ46_RS10505	2259337..2260377	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ46_RS10510	2260619..2261338	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ46_RS10515	2261328..2261744	+	417	WP_003410114.1	hypothetical protein	-
FPJ46_RS10520	2261760..2262059	-	300	WP_003410120.1	hypothetical protein	-
FPJ46_RS10525	2262144..2262449	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ46_RS10530	2262458..2263063	-	606	WP_031751732.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ46_RS10535	2263088..2263447	-	360	WP_003410131.1	hypothetical protein	-
FPJ46_RS10540	2263607..2264284	-	678	WP_003905725.1	hypothetical protein	-
FPJ46_RS10545	2264236..2264472	-	237	WP_003410133.1	hypothetical protein	-
FPJ46_RS10550	2264679..2265575	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22686.92 Da
• Isoelectric Point: 7.6271

>T133635 WP_031751732.1 NZ_CP041860:c2263063-2262458 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRATYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T133635 NZ_CP041860:c2263063-2262458 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGCGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT133635 WP_003410124.1 NZ_CP041860:c2262449-2262144 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT133635 NZ_CP041860:c2262449-2262144 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV30