Detailed information of TA system TA133593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3558643..3559313
• Replicon: chromosome
• Accession: NZ_CP041859
• Organism: Mycobacterium tuberculosis strain 1-0061P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPJ47_RS16625
• Protein ID: WP_003899954.1
• Coordinates: 3558643..3558987 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPJ47_RS16630
• Protein ID: WP_003899955.1
• Coordinates: 3558984..3559313 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ47_RS16595	3553716..3554576	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPJ47_RS16600	3554551..3555066	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPJ47_RS16605	3555306..3555599	+	294	WP_003416635.1	hypothetical protein	-
FPJ47_RS16610	3555887..3557176	+	1290	WP_003416640.1	ATP-binding protein	-
FPJ47_RS16615	3557523..3557957	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPJ47_RS16620	3557960..3558412	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ47_RS16625	3558643..3558987	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ47_RS16630	3558984..3559313	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPJ47_RS16635	3559851..3560198	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPJ47_RS16640	3560195..3560815	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPJ47_RS16645	3560975..3562240	-	1266	WP_003909837.1	hypothetical protein	-
FPJ47_RS16650	3562408..3562617	+	210	WP_003416778.1	hypothetical protein	-
FPJ47_RS16655	3562864..3563898	-	1035	WP_003905011.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T133593 WP_003899954.1 NZ_CP041859:3558643-3558987 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T133593 NZ_CP041859:3558643-3558987 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT133593 WP_003899955.1 NZ_CP041859:3558984-3559313 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT133593 NZ_CP041859:3558984-3559313 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6