Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3558643..3559313 Replicon chromosome
Accession NZ_CP041859
Organism Mycobacterium tuberculosis strain 1-0061P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ47_RS16625 Protein ID WP_003899954.1
Coordinates 3558643..3558987 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ47_RS16630 Protein ID WP_003899955.1
Coordinates 3558984..3559313 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ47_RS16595 3553716..3554576 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ47_RS16600 3554551..3555066 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ47_RS16605 3555306..3555599 + 294 WP_003416635.1 hypothetical protein -
FPJ47_RS16610 3555887..3557176 + 1290 WP_003416640.1 ATP-binding protein -
FPJ47_RS16615 3557523..3557957 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ47_RS16620 3557960..3558412 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ47_RS16625 3558643..3558987 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ47_RS16630 3558984..3559313 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ47_RS16635 3559851..3560198 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPJ47_RS16640 3560195..3560815 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FPJ47_RS16645 3560975..3562240 - 1266 WP_003909837.1 hypothetical protein -
FPJ47_RS16650 3562408..3562617 + 210 WP_003416778.1 hypothetical protein -
FPJ47_RS16655 3562864..3563898 - 1035 WP_003905011.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T133593 WP_003899954.1 NZ_CP041859:3558643-3558987 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T133593 NZ_CP041859:3558643-3558987 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT133593 WP_003899955.1 NZ_CP041859:3558984-3559313 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT133593 NZ_CP041859:3558984-3559313 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References