Detailed information of TA system TA133576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2280452..2281371
• Replicon: chromosome
• Accession: NZ_CP041859
• Organism: Mycobacterium tuberculosis strain 1-0061P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: FPJ47_RS10585
• Protein ID: WP_003900449.1
• Coordinates: 2280766..2281371 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ47_RS10580
• Protein ID: WP_003410124.1
• Coordinates: 2280452..2280757 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ47_RS10550	2275463..2276719	-	1257	WP_003902247.1	HNH endonuclease	-
FPJ47_RS10555	2277073..2277648	+	576	WP_003410100.1	hypothetical protein	-
FPJ47_RS10560	2277645..2278685	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ47_RS10565	2278927..2279646	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ47_RS10570	2279636..2280052	+	417	WP_003410114.1	hypothetical protein	-
FPJ47_RS10575	2280068..2280367	-	300	WP_003410120.1	hypothetical protein	-
FPJ47_RS10580	2280452..2280757	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ47_RS10585	2280766..2281371	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ47_RS10590	2281396..2281755	-	360	WP_003410131.1	hypothetical protein	-
FPJ47_RS10595	2281915..2282592	-	678	WP_003900450.1	hypothetical protein	-
FPJ47_RS10600	2282544..2282780	-	237	WP_003410133.1	hypothetical protein	-
FPJ47_RS10605	2282987..2283883	-	897	WP_003904759.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T133576 WP_003900449.1 NZ_CP041859:c2281371-2280766 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T133576 NZ_CP041859:c2281371-2280766 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT133576 WP_003410124.1 NZ_CP041859:c2280757-2280452 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT133576 NZ_CP041859:c2280757-2280452 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30