Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3567114..3567784 Replicon chromosome
Accession NZ_CP041838
Organism Mycobacterium tuberculosis strain 2-0028P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ70_RS16685 Protein ID WP_003899954.1
Coordinates 3567114..3567458 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ70_RS16690 Protein ID WP_003899955.1
Coordinates 3567455..3567784 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ70_RS16655 3562187..3563047 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ70_RS16660 3563022..3563537 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ70_RS16665 3563777..3564070 + 294 WP_003416635.1 hypothetical protein -
FPJ70_RS16670 3564358..3565647 + 1290 WP_003416640.1 ATP-binding protein -
FPJ70_RS16675 3565994..3566428 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ70_RS16680 3566431..3566883 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ70_RS16685 3567114..3567458 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ70_RS16690 3567455..3567784 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ70_RS16695 3567868..3569129 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FPJ70_RS16700 3569680..3570027 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPJ70_RS16705 3570024..3570644 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FPJ70_RS16710 3570804..3572069 - 1266 WP_003902423.1 hypothetical protein -
FPJ70_RS16715 3572237..3572446 + 210 WP_003416778.1 hypothetical protein -
FPJ70_RS16720 3572481..3572666 + 186 WP_003908177.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T132330 WP_003899954.1 NZ_CP041838:3567114-3567458 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T132330 NZ_CP041838:3567114-3567458 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT132330 WP_003899955.1 NZ_CP041838:3567455-3567784 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT132330 NZ_CP041838:3567455-3567784 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References