Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2191070..2191938 Replicon chromosome
Accession NZ_CP041830
Organism Mycobacterium tuberculosis strain 2-0068P6C4

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FPJ78_RS10125 Protein ID WP_010886136.1
Coordinates 2191070..2191447 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FPJ78_RS10130 Protein ID WP_003409886.1
Coordinates 2191489..2191938 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ78_RS10075 2186411..2186593 - 183 WP_003409870.1 hypothetical protein -
FPJ78_RS10080 2186707..2187057 - 351 WP_003409871.1 hypothetical protein -
FPJ78_RS10085 2187068..2187556 - 489 Protein_1992 hypothetical protein -
FPJ78_RS10090 2187608..2188869 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FPJ78_RS10095 2188912..2189328 - 417 Protein_1994 hypothetical protein -
FPJ78_RS10100 2189349..2189540 - 192 WP_003409876.1 hypothetical protein -
FPJ78_RS10105 2189541..2189837 - 297 WP_003409877.1 hypothetical protein -
FPJ78_RS10110 2190077..2190292 + 216 WP_003409878.1 antitoxin -
FPJ78_RS10115 2190289..2190600 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FPJ78_RS10120 2190574..2191095 - 522 WP_003904745.1 hypothetical protein -
FPJ78_RS10125 2191070..2191447 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FPJ78_RS10130 2191489..2191938 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FPJ78_RS10135 2191935..2192480 + 546 WP_003409891.1 SecB-like chaperone -
FPJ78_RS10140 2192369..2192983 - 615 WP_003901296.1 hypothetical protein -
FPJ78_RS10145 2193032..2193328 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FPJ78_RS10150 2193325..2193576 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FPJ78_RS10155 2193563..2194057 + 495 WP_003899099.1 hypothetical protein -
FPJ78_RS10160 2194217..2194624 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FPJ78_RS10165 2194628..2194900 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ78_RS10170 2194933..2196153 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T131821 WP_010886136.1 NZ_CP041830:2191070-2191447 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T131821 NZ_CP041830:2191070-2191447 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT131821 WP_003409886.1 NZ_CP041830:2191489-2191938 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT131821 NZ_CP041830:2191489-2191938 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References