Detailed information of TA system TA131768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2252149..2253068
• Replicon: chromosome
• Accession: NZ_CP041829
• Organism: Mycobacterium tuberculosis strain 3-0059P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: FPJ79_RS10495
• Protein ID: WP_003906719.1
• Coordinates: 2252463..2253068 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ79_RS10490
• Protein ID: WP_003410124.1
• Coordinates: 2252149..2252454 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ79_RS10460	2247160..2248416	-	1257	WP_003410095.1	HNH endonuclease	-
FPJ79_RS10465	2248770..2249345	+	576	WP_003410100.1	hypothetical protein	-
FPJ79_RS10470	2249342..2250382	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ79_RS10475	2250624..2251343	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ79_RS10480	2251333..2251749	+	417	WP_003410114.1	hypothetical protein	-
FPJ79_RS10485	2251765..2252064	-	300	WP_003410120.1	hypothetical protein	-
FPJ79_RS10490	2252149..2252454	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ79_RS10495	2252463..2253068	-	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ79_RS10500	2253093..2253452	-	360	WP_003410131.1	hypothetical protein	-
FPJ79_RS10505	2253612..2254289	-	678	WP_003900450.1	hypothetical protein	-
FPJ79_RS10510	2254241..2254477	-	237	WP_003410133.1	hypothetical protein	-
FPJ79_RS10515	2254684..2255580	-	897	WP_003906720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T131768 WP_003906719.1 NZ_CP041829:c2253068-2252463 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T131768 NZ_CP041829:c2253068-2252463 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGCGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT131768 WP_003410124.1 NZ_CP041829:c2252454-2252149 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT131768 NZ_CP041829:c2252454-2252149 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A654U351

Antitoxin

• AlphaFold DB: A0A7U4BV30