Detailed information of TA system TA131402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2153876..2154795
• Replicon: chromosome
• Accession: NZ_CP041823
• Organism: Mycobacterium tuberculosis strain 4-0019P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: FPJ85_RS10190
• Protein ID: WP_003906719.1
• Coordinates: 2153876..2154481 (+)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ85_RS10195
• Protein ID: WP_003410124.1
• Coordinates: 2154490..2154795 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ85_RS10170	2151364..2152260	+	897	WP_003906720.1	hypothetical protein	-
FPJ85_RS10175	2152467..2152703	+	237	WP_003410133.1	hypothetical protein	-
FPJ85_RS10180	2152655..2153332	+	678	WP_003900450.1	hypothetical protein	-
FPJ85_RS10185	2153492..2153851	+	360	WP_003410131.1	hypothetical protein	-
FPJ85_RS10190	2153876..2154481	+	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ85_RS10195	2154490..2154795	+	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ85_RS10200	2154880..2155179	+	300	WP_003410120.1	hypothetical protein	-
FPJ85_RS10205	2155195..2155497	-	303	WP_003899135.1	hypothetical protein	-
FPJ85_RS10215	2156959..2157678	-	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ85_RS10220	2157920..2158960	-	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ85_RS10225	2158957..2159532	-	576	WP_003410100.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T131402 WP_003906719.1 NZ_CP041823:2153876-2154481 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T131402 NZ_CP041823:2153876-2154481 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGCGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT131402 WP_003410124.1 NZ_CP041823:2154490-2154795 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT131402 NZ_CP041823:2154490-2154795 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A654U351

Antitoxin

• AlphaFold DB: A0A7U4BV30