Detailed information of TA system TA131222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2275001..2275920
• Replicon: chromosome
• Accession: NZ_CP041820
• Organism: Mycobacterium tuberculosis strain 4-0073P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: FPJ89_RS10580
• Protein ID: WP_003900449.1
• Coordinates: 2275315..2275920 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ89_RS10575
• Protein ID: WP_003410124.1
• Coordinates: 2275001..2275306 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ89_RS10545	2270381..2271268	-	888	Protein_2084	13E12 repeat family protein	-
FPJ89_RS10550	2271622..2272197	+	576	WP_003410100.1	hypothetical protein	-
FPJ89_RS10555	2272194..2273234	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ89_RS10560	2273476..2274195	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ89_RS10565	2274185..2274601	+	417	WP_003410114.1	hypothetical protein	-
FPJ89_RS10570	2274617..2274916	-	300	WP_003410120.1	hypothetical protein	-
FPJ89_RS10575	2275001..2275306	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ89_RS10580	2275315..2275920	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ89_RS10585	2275945..2276304	-	360	WP_003410131.1	hypothetical protein	-
FPJ89_RS10590	2276464..2277141	-	678	WP_003900450.1	hypothetical protein	-
FPJ89_RS10595	2277093..2277329	-	237	WP_003410133.1	hypothetical protein	-
FPJ89_RS10600	2277536..2278432	-	897	WP_003906720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T131222 WP_003900449.1 NZ_CP041820:c2275920-2275315 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T131222 NZ_CP041820:c2275920-2275315 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT131222 WP_003410124.1 NZ_CP041820:c2275306-2275001 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT131222 NZ_CP041820:c2275306-2275001 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30