Detailed information of TA system TA131118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3555291..3555985
• Replicon: chromosome
• Accession: NZ_CP041818
• Organism: Mycobacterium tuberculosis strain 4-0087P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPJ91_RS16625
• Protein ID: WP_003899954.1
• Coordinates: 3555291..3555635 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FPJ91_RS16630
• Protein ID: WP_017487555.1
• Coordinates: 3555632..3555985 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ91_RS16595	3550364..3551224	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPJ91_RS16600	3551199..3551714	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPJ91_RS16605	3551954..3552247	+	294	WP_003416635.1	hypothetical protein	-
FPJ91_RS16610	3552535..3553824	+	1290	WP_003416640.1	ATP-binding protein	-
FPJ91_RS16615	3554171..3554605	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPJ91_RS16620	3554608..3555060	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ91_RS16625	3555291..3555635	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ91_RS16630	3555632..3555985	+	354	WP_017487555.1	XRE family transcriptional regulator	Antitoxin
FPJ91_RS16640	3557858..3558205	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPJ91_RS16645	3558202..3558822	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPJ91_RS16650	3558982..3560247	-	1266	WP_003899958.1	hypothetical protein	-
FPJ91_RS16655	3560415..3560624	+	210	WP_003416778.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T131118 WP_003899954.1 NZ_CP041818:3555291-3555635 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T131118 NZ_CP041818:3555291-3555635 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 12613.32 Da
• Isoelectric Point: 7.4053

>AT131118 WP_017487555.1 NZ_CP041818:3555632-3555985 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVVNRPGESGDSLI

Nucleotide

• Length: 354 bp

>AT131118 NZ_CP041818:3555632-3555985 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGTGAA
CCGCCCCGGTGAGTCCGGAGACTCTCTGATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2