Detailed information of TA system TA131057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3543156..3543826
• Replicon: chromosome
• Accession: NZ_CP041817
• Organism: Mycobacterium tuberculosis strain 4-0096P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPJ92_RS16610
• Protein ID: WP_003899954.1
• Coordinates: 3543156..3543500 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPJ92_RS16615
• Protein ID: WP_003899955.1
• Coordinates: 3543497..3543826 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ92_RS16580	3538229..3539089	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPJ92_RS16585	3539064..3539579	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPJ92_RS16590	3539819..3540112	+	294	WP_019250183.1	hypothetical protein	-
FPJ92_RS16595	3540400..3541689	+	1290	WP_003416640.1	ATP-binding protein	-
FPJ92_RS16600	3542036..3542470	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPJ92_RS16605	3542473..3542925	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ92_RS16610	3543156..3543500	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ92_RS16615	3543497..3543826	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPJ92_RS16620	3544364..3544711	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPJ92_RS16625	3544708..3545328	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPJ92_RS16630	3545488..3546753	-	1266	WP_003902423.1	hypothetical protein	-
FPJ92_RS16635	3546921..3547130	+	210	WP_003416778.1	hypothetical protein	-
FPJ92_RS16640	3547377..3548411	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T131057 WP_003899954.1 NZ_CP041817:3543156-3543500 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T131057 NZ_CP041817:3543156-3543500 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT131057 WP_003899955.1 NZ_CP041817:3543497-3543826 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT131057 NZ_CP041817:3543497-3543826 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCGGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6