Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2257606..2258525 Replicon chromosome
Accession NZ_CP041817
Organism Mycobacterium tuberculosis strain 4-0096P6C4

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag FPJ92_RS10505 Protein ID WP_003900449.1
Coordinates 2257920..2258525 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag FPJ92_RS10500 Protein ID WP_003410124.1
Coordinates 2257606..2257911 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ92_RS10470 2252930..2253520 + 591 WP_003899131.1 IS110 family transposase -
FPJ92_RS10475 2253648..2254904 - 1257 WP_003902247.1 HNH endonuclease -
FPJ92_RS10480 2255258..2255614 + 357 Protein_2071 hypothetical protein -
FPJ92_RS10490 2256967..2257206 + 240 Protein_2073 hypothetical protein -
FPJ92_RS10495 2257222..2257521 - 300 WP_003410120.1 hypothetical protein -
FPJ92_RS10500 2257606..2257911 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
FPJ92_RS10505 2257920..2258525 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ92_RS10510 2258550..2258909 - 360 WP_003410131.1 hypothetical protein -
FPJ92_RS10515 2259069..2259746 - 678 WP_003902245.1 hypothetical protein -
FPJ92_RS10520 2259698..2259934 - 237 WP_003410133.1 hypothetical protein -
FPJ92_RS10525 2260141..2261037 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T131040 WP_003900449.1 NZ_CP041817:c2258525-2257920 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T131040 NZ_CP041817:c2258525-2257920 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT131040 WP_003410124.1 NZ_CP041817:c2257911-2257606 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT131040 NZ_CP041817:c2257911-2257606 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References