Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3554271..3554941 Replicon chromosome
Accession NZ_CP041816
Organism Mycobacterium tuberculosis strain SEA00042P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ93_RS16620 Protein ID WP_003899954.1
Coordinates 3554271..3554615 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ93_RS16625 Protein ID WP_003899955.1
Coordinates 3554612..3554941 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ93_RS16590 3549344..3550204 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ93_RS16595 3550179..3550694 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ93_RS16600 3550934..3551227 + 294 WP_003416635.1 hypothetical protein -
FPJ93_RS16605 3551515..3552804 + 1290 WP_003416640.1 ATP-binding protein -
FPJ93_RS16610 3553151..3553585 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ93_RS16615 3553588..3554040 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ93_RS16620 3554271..3554615 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ93_RS16625 3554612..3554941 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ93_RS16630 3555479..3555826 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPJ93_RS16635 3555823..3556443 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FPJ93_RS16640 3556603..3557331 - 729 Protein_3285 hypothetical protein -
FPJ93_RS16650 3558914..3559123 + 210 WP_003416778.1 hypothetical protein -
FPJ93_RS16655 3559158..3559343 + 186 WP_003908177.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T130997 WP_003899954.1 NZ_CP041816:3554271-3554615 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T130997 NZ_CP041816:3554271-3554615 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT130997 WP_003899955.1 NZ_CP041816:3554612-3554941 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT130997 NZ_CP041816:3554612-3554941 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGCGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References