Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3554757..3555427 Replicon chromosome
Accession NZ_CP041814
Organism Mycobacterium tuberculosis strain SEA06535P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPJ95_RS16615 Protein ID WP_003899954.1
Coordinates 3554757..3555101 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPJ95_RS16620 Protein ID WP_003899955.1
Coordinates 3555098..3555427 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ95_RS16585 3549830..3550690 + 861 WP_003416628.1 alpha/beta hydrolase -
FPJ95_RS16590 3550665..3551180 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPJ95_RS16595 3551420..3551713 + 294 WP_003416635.1 hypothetical protein -
FPJ95_RS16600 3552001..3553290 + 1290 WP_003416640.1 ATP-binding protein -
FPJ95_RS16605 3553637..3554071 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPJ95_RS16610 3554074..3554526 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPJ95_RS16615 3554757..3555101 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ95_RS16620 3555098..3555427 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPJ95_RS16625 3555965..3556312 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPJ95_RS16630 3556309..3556929 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
FPJ95_RS16635 3557089..3558354 - 1266 WP_003899958.1 hypothetical protein -
FPJ95_RS16640 3558522..3558731 + 210 WP_003416778.1 hypothetical protein -
FPJ95_RS16645 3558978..3560012 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T130876 WP_003899954.1 NZ_CP041814:3554757-3555101 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T130876 NZ_CP041814:3554757-3555101 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT130876 WP_003899955.1 NZ_CP041814:3555098-3555427 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT130876 NZ_CP041814:3555098-3555427 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References