Detailed information of TA system TA130737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2261889..2262808
• Replicon: chromosome
• Accession: NZ_CP041812
• Organism: Mycobacterium tuberculosis strain SEA07020250P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: FPJ97_RS10545
• Protein ID: WP_003906719.1
• Coordinates: 2262203..2262808 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ97_RS10540
• Protein ID: WP_003410124.1
• Coordinates: 2261889..2262194 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ97_RS10510	2257920..2258156	-	237	Protein_2077	DUF222 domain-containing protein	-
FPJ97_RS10515	2258510..2259085	+	576	WP_003410100.1	hypothetical protein	-
FPJ97_RS10520	2259082..2260122	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ97_RS10525	2260364..2261083	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ97_RS10530	2261073..2261489	+	417	WP_003410114.1	hypothetical protein	-
FPJ97_RS10535	2261505..2261804	-	300	WP_003410120.1	hypothetical protein	-
FPJ97_RS10540	2261889..2262194	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ97_RS10545	2262203..2262808	-	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ97_RS10550	2262833..2263192	-	360	WP_003410131.1	hypothetical protein	-
FPJ97_RS10555	2263352..2264029	-	678	WP_003900450.1	hypothetical protein	-
FPJ97_RS10560	2263981..2264217	-	237	WP_003410133.1	hypothetical protein	-
FPJ97_RS10565	2264424..2265320	-	897	WP_003906720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T130737 WP_003906719.1 NZ_CP041812:c2262808-2262203 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T130737 NZ_CP041812:c2262808-2262203 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGCGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT130737 WP_003410124.1 NZ_CP041812:c2262194-2261889 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT130737 NZ_CP041812:c2262194-2261889 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A654U351

Antitoxin

• AlphaFold DB: A0A7U4BV30