Detailed information of TA system TA130633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3545927..3546597
• Replicon: chromosome
• Accession: NZ_CP041810
• Organism: Mycobacterium tuberculosis strain SEA08162P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPJ99_RS16630
• Protein ID: WP_003899954.1
• Coordinates: 3545927..3546271 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPJ99_RS16635
• Protein ID: WP_003899955.1
• Coordinates: 3546268..3546597 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ99_RS16600	3541000..3541860	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPJ99_RS16605	3541835..3542350	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPJ99_RS16610	3542590..3542883	+	294	WP_003416635.1	hypothetical protein	-
FPJ99_RS16615	3543171..3544460	+	1290	WP_003906996.1	ATP-binding protein	-
FPJ99_RS16620	3544807..3545241	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPJ99_RS16625	3545244..3545696	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPJ99_RS16630	3545927..3546271	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ99_RS16635	3546268..3546597	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPJ99_RS16640	3547135..3547482	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPJ99_RS16645	3547479..3548099	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPJ99_RS16650	3548259..3549524	-	1266	WP_003902423.1	hypothetical protein	-
FPJ99_RS16655	3549692..3549901	+	210	WP_003416778.1	hypothetical protein	-
FPJ99_RS16660	3550148..3551182	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T130633 WP_003899954.1 NZ_CP041810:3545927-3546271 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T130633 NZ_CP041810:3545927-3546271 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT130633 WP_003899955.1 NZ_CP041810:3546268-3546597 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT130633 NZ_CP041810:3546268-3546597 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6