Detailed information of TA system TA130615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2262686..2263605
• Replicon: chromosome
• Accession: NZ_CP041810
• Organism: Mycobacterium tuberculosis strain SEA08162P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A654U351
• Locus tag: FPJ99_RS10535
• Protein ID: WP_003906719.1
• Coordinates: 2263000..2263605 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPJ99_RS10530
• Protein ID: WP_003410124.1
• Coordinates: 2262686..2262991 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ99_RS10500	2257697..2258953	-	1257	WP_003410095.1	HNH endonuclease	-
FPJ99_RS10505	2259307..2259882	+	576	WP_003410100.1	hypothetical protein	-
FPJ99_RS10510	2259879..2260919	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPJ99_RS10515	2261161..2261880	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPJ99_RS10520	2261870..2262286	+	417	WP_003410114.1	hypothetical protein	-
FPJ99_RS10525	2262302..2262601	-	300	WP_003410120.1	hypothetical protein	-
FPJ99_RS10530	2262686..2262991	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPJ99_RS10535	2263000..2263605	-	606	WP_003906719.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPJ99_RS10540	2263630..2263989	-	360	WP_003410131.1	hypothetical protein	-
FPJ99_RS10545	2264149..2264826	-	678	WP_003900450.1	hypothetical protein	-
FPJ99_RS10550	2264778..2265014	-	237	WP_003410133.1	hypothetical protein	-
FPJ99_RS10555	2265221..2266117	-	897	WP_003906720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22716.91 Da
• Isoelectric Point: 7.3073

>T130615 WP_003906719.1 NZ_CP041810:c2263605-2263000 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLADTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T130615 NZ_CP041810:c2263605-2263000 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGCGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT130615 WP_003410124.1 NZ_CP041810:c2262991-2262686 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT130615 NZ_CP041810:c2262991-2262686 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A654U351

Antitoxin

• AlphaFold DB: A0A7U4BV30