Detailed information of TA system TA130208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3548281..3548951
• Replicon: chromosome
• Accession: NZ_CP041803
• Organism: Mycobacterium tuberculosis strain SEA11278P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPK06_RS16540
• Protein ID: WP_003899954.1
• Coordinates: 3548281..3548625 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPK06_RS16545
• Protein ID: WP_003899955.1
• Coordinates: 3548622..3548951 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK06_RS16510	3543354..3544214	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPK06_RS16515	3544189..3544704	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPK06_RS16520	3544944..3545237	+	294	WP_003416635.1	hypothetical protein	-
FPK06_RS16525	3545525..3546814	+	1290	WP_003416640.1	ATP-binding protein	-
FPK06_RS16530	3547161..3547595	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPK06_RS16535	3547598..3548050	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPK06_RS16540	3548281..3548625	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPK06_RS16545	3548622..3548951	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPK06_RS16550	3549489..3549836	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPK06_RS16555	3549833..3550453	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPK06_RS16560	3550613..3551878	-	1266	WP_003909837.1	hypothetical protein	-
FPK06_RS16565	3552046..3552255	+	210	WP_003416778.1	hypothetical protein	-
FPK06_RS16570	3552502..3553536	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T130208 WP_003899954.1 NZ_CP041803:3548281-3548625 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T130208 NZ_CP041803:3548281-3548625 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT130208 WP_003899955.1 NZ_CP041803:3548622-3548951 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT130208 NZ_CP041803:3548622-3548951 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6