Detailed information of TA system TA130191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2274683..2275602
• Replicon: chromosome
• Accession: NZ_CP041803
• Organism: Mycobacterium tuberculosis strain SEA11278P6C4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: FPK06_RS10525
• Protein ID: WP_003900449.1
• Coordinates: 2274997..2275602 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: FPK06_RS10520
• Protein ID: WP_003410124.1
• Coordinates: 2274683..2274988 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK06_RS10490	2269694..2270950	-	1257	WP_003410095.1	HNH endonuclease	-
FPK06_RS10495	2271304..2271879	+	576	WP_003410100.1	hypothetical protein	-
FPK06_RS10500	2271876..2272916	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
FPK06_RS10505	2273158..2273877	+	720	WP_003410108.1	DUF433 domain-containing protein	-
FPK06_RS10510	2273867..2274283	+	417	WP_003410114.1	hypothetical protein	-
FPK06_RS10515	2274299..2274598	-	300	WP_003410120.1	hypothetical protein	-
FPK06_RS10520	2274683..2274988	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
FPK06_RS10525	2274997..2275602	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPK06_RS10530	2275627..2275986	-	360	WP_003410131.1	hypothetical protein	-
FPK06_RS10535	2276146..2276823	-	678	WP_003900450.1	hypothetical protein	-
FPK06_RS10540	2276775..2277011	-	237	WP_003410133.1	hypothetical protein	-
FPK06_RS10545	2277218..2278114	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T130191 WP_003900449.1 NZ_CP041803:c2275602-2274997 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T130191 NZ_CP041803:c2275602-2274997 [Mycobacterium tuberculosis]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT130191 WP_003410124.1 NZ_CP041803:c2274988-2274683 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT130191 NZ_CP041803:c2274988-2274683 [Mycobacterium tuberculosis]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCATTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30