Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2203835..2204703 Replicon chromosome
Accession NZ_CP041802
Organism Mycobacterium tuberculosis strain SEA12126P6C4

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FPK07_RS10125 Protein ID WP_010886136.1
Coordinates 2203835..2204212 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FPK07_RS10130 Protein ID WP_003409886.1
Coordinates 2204254..2204703 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK07_RS10075 2199624..2200076 - 453 WP_003899095.1 lipoprotein -
FPK07_RS10080 2200140..2200541 + 402 WP_003409869.1 hypothetical protein -
FPK07_RS10085 2200534..2200716 - 183 WP_003409870.1 hypothetical protein -
FPK07_RS10090 2200830..2201180 - 351 WP_003409871.1 hypothetical protein -
FPK07_RS10095 2201191..2202093 - 903 WP_003409874.1 hypothetical protein -
FPK07_RS10100 2202114..2202305 - 192 WP_003409876.1 hypothetical protein -
FPK07_RS10105 2202306..2202602 - 297 WP_003409877.1 hypothetical protein -
FPK07_RS10110 2202842..2203057 + 216 WP_003409878.1 antitoxin -
FPK07_RS10115 2203054..2203365 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FPK07_RS10120 2203339..2203860 - 522 WP_003904745.1 hypothetical protein -
FPK07_RS10125 2203835..2204212 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FPK07_RS10130 2204254..2204703 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FPK07_RS10135 2204700..2205245 + 546 WP_031663732.1 hypothetical protein -
FPK07_RS10140 2205134..2205748 - 615 WP_003901296.1 hypothetical protein -
FPK07_RS10145 2205797..2206093 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FPK07_RS10150 2206090..2206341 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FPK07_RS10155 2206328..2206822 + 495 WP_003899099.1 hypothetical protein -
FPK07_RS10160 2206982..2207389 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FPK07_RS10165 2207393..2207665 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPK07_RS10170 2207698..2208918 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T130125 WP_010886136.1 NZ_CP041802:2203835-2204212 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T130125 NZ_CP041802:2203835-2204212 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT130125 WP_003409886.1 NZ_CP041802:2204254-2204703 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT130125 NZ_CP041802:2204254-2204703 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References