Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3535818..3536488 Replicon chromosome
Accession NZ_CP041797
Organism Mycobacterium tuberculosis strain SEA14117P6C4

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag FPK12_RS16575 Protein ID WP_003899954.1
Coordinates 3535818..3536162 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag FPK12_RS16580 Protein ID WP_003899955.1
Coordinates 3536159..3536488 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK12_RS16545 3530891..3531751 + 861 WP_003416628.1 alpha/beta hydrolase -
FPK12_RS16550 3531726..3532241 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
FPK12_RS16555 3532481..3532774 + 294 WP_003416635.1 hypothetical protein -
FPK12_RS16560 3533062..3534351 + 1290 WP_003416640.1 ATP-binding protein -
FPK12_RS16565 3534698..3535132 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
FPK12_RS16570 3535135..3535587 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPK12_RS16575 3535818..3536162 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPK12_RS16580 3536159..3536488 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
FPK12_RS16585 3536719..3537980 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FPK12_RS16590 3538385..3538732 + 348 WP_003899956.1 DUF2384 domain-containing protein -
FPK12_RS16595 3538729..3539349 + 621 WP_038338382.1 RES family NAD+ phosphorylase -
FPK12_RS16600 3539509..3539733 - 225 Protein_3277 hypothetical protein -
FPK12_RS16605 3539785..3541046 + 1262 WP_193018814.1 IS3-like element IS987 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T129850 WP_003899954.1 NZ_CP041797:3535818-3536162 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T129850 NZ_CP041797:3535818-3536162 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT129850 WP_003899955.1 NZ_CP041797:3536159-3536488 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT129850 NZ_CP041797:3536159-3536488 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References