Detailed information of TA system TA129789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3562204..3562874
• Replicon: chromosome
• Accession: NZ_CP041796
• Organism: Mycobacterium tuberculosis strain SEA14318P6C4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: FPK13_RS16645
• Protein ID: WP_003899954.1
• Coordinates: 3562204..3562548 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: FPK13_RS16650
• Protein ID: WP_003899955.1
• Coordinates: 3562545..3562874 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPK13_RS16615	3557277..3558137	+	861	WP_003416628.1	alpha/beta hydrolase	-
FPK13_RS16620	3558112..3558627	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
FPK13_RS16625	3558867..3559160	+	294	WP_003416635.1	hypothetical protein	-
FPK13_RS16630	3559448..3560737	+	1290	WP_003416640.1	ATP-binding protein	-
FPK13_RS16635	3561084..3561518	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
FPK13_RS16640	3561521..3561973	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FPK13_RS16645	3562204..3562548	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FPK13_RS16650	3562545..3562874	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
FPK13_RS16655	3563412..3563759	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
FPK13_RS16660	3563756..3564376	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
FPK13_RS16665	3564536..3565801	-	1266	WP_003909837.1	hypothetical protein	-
FPK13_RS16670	3565969..3566178	+	210	WP_003416778.1	hypothetical protein	-
FPK13_RS16675	3566425..3567459	-	1035	WP_003905011.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T129789 WP_003899954.1 NZ_CP041796:3562204-3562548 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T129789 NZ_CP041796:3562204-3562548 [Mycobacterium tuberculosis]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT129789 WP_003899955.1 NZ_CP041796:3562545-3562874 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT129789 NZ_CP041796:3562545-3562874 [Mycobacterium tuberculosis]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6