Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2204258..2205126 Replicon chromosome
Accession NZ_CP041795
Organism Mycobacterium tuberculosis strain SEA14333P6C4

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag FPK14_RS10125 Protein ID WP_010886136.1
Coordinates 2204258..2204635 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag FPK14_RS10130 Protein ID WP_003409886.1
Coordinates 2204677..2205126 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPK14_RS10075 2200047..2200499 - 453 WP_003899095.1 lipoprotein -
FPK14_RS10080 2200563..2200964 + 402 WP_003409869.1 hypothetical protein -
FPK14_RS10085 2200957..2201139 - 183 WP_003409870.1 hypothetical protein -
FPK14_RS10090 2201253..2201603 - 351 WP_003409871.1 hypothetical protein -
FPK14_RS10095 2201614..2202516 - 903 WP_003409874.1 hypothetical protein -
FPK14_RS10100 2202537..2202728 - 192 WP_003409876.1 hypothetical protein -
FPK14_RS10105 2202729..2203025 - 297 WP_003409877.1 hypothetical protein -
FPK14_RS10110 2203265..2203480 + 216 WP_003409878.1 antitoxin -
FPK14_RS10115 2203477..2203788 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
FPK14_RS10120 2203762..2204283 - 522 WP_003904745.1 hypothetical protein -
FPK14_RS10125 2204258..2204635 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
FPK14_RS10130 2204677..2205126 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
FPK14_RS10135 2205123..2205668 + 546 WP_031663732.1 hypothetical protein -
FPK14_RS10140 2205557..2206171 - 615 WP_003901296.1 hypothetical protein -
FPK14_RS10145 2206220..2206516 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
FPK14_RS10150 2206513..2206764 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
FPK14_RS10155 2206751..2207245 + 495 WP_003899099.1 hypothetical protein -
FPK14_RS10160 2207405..2207812 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
FPK14_RS10165 2207816..2208088 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FPK14_RS10170 2208121..2209341 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T129705 WP_010886136.1 NZ_CP041795:2204258-2204635 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T129705 NZ_CP041795:2204258-2204635 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT129705 WP_003409886.1 NZ_CP041795:2204677-2205126 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT129705 NZ_CP041795:2204677-2205126 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References