Detailed information of TA system TA129230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5783874..5784469
• Replicon: chromosome
• Accession: NZ_CP041774
• Organism: Pseudomonas aeruginosa strain 60503

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9E8L6K5
• Locus tag: FOY97_RS27970
• Protein ID: WP_071534386.1
• Coordinates: 5784191..5784469 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOY97_RS27965
• Protein ID: WP_003133769.1
• Coordinates: 5783874..5784179 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY97_RS27950	5779662..5780336	-	675	WP_079381483.1	DUF2290 domain-containing protein	-
FOY97_RS27955	5780333..5782540	-	2208	WP_079381482.1	DEAD/DEAH box helicase	-
FOY97_RS27960	5782948..5783538	-	591	WP_108703096.1	ankyrin repeat domain-containing protein	-
FOY97_RS27965	5783874..5784179	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
FOY97_RS27970	5784191..5784469	-	279	WP_071534386.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOY97_RS27980	5784792..5787020	+	2229	WP_033938414.1	TonB-dependent receptor	-
FOY97_RS27985	5787090..5787737	-	648	WP_003095021.1	carbonate dehydratase	-
FOY97_RS27990	5787799..5789037	-	1239	WP_033938412.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10698.23 Da
• Isoelectric Point: 7.9184

>T129230 WP_071534386.1 NZ_CP041774:c5784469-5784191 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMHHAATELRDLRSPPGNRLEPLQGKRVGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T129230 NZ_CP041774:c5784469-5784191 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCATCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGTGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT129230 WP_003133769.1 NZ_CP041774:c5784179-5783874 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT129230 NZ_CP041774:c5784179-5783874 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTACGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCCCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG