Detailed information of TA system TA129220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1356070..1356665
• Replicon: chromosome
• Accession: NZ_CP041773
• Organism: Pseudomonas aeruginosa strain 519119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: FOY95_RS06645
• Protein ID: WP_003117425.1
• Coordinates: 1356387..1356665 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOY95_RS06640
• Protein ID: WP_003113527.1
• Coordinates: 1356070..1356375 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY95_RS06605	1351210..1352058	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
FOY95_RS06615	1352225..1353166	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
FOY95_RS06620	1353283..1353897	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
FOY95_RS06625	1353939..1354523	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
FOY95_RS06630	1354564..1355664	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
FOY95_RS06640	1356070..1356375	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
FOY95_RS06645	1356387..1356665	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOY95_RS06655	1356994..1359222	+	2229	WP_004352671.1	TonB-dependent receptor	-
FOY95_RS06660	1359292..1359939	-	648	WP_003095021.1	carbonate dehydratase	-
FOY95_RS06665	1360001..1361239	-	1239	WP_023123442.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T129220 WP_003117425.1 NZ_CP041773:c1356665-1356387 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T129220 NZ_CP041773:c1356665-1356387 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT129220 WP_003113527.1 NZ_CP041773:c1356375-1356070 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT129220 NZ_CP041773:c1356375-1356070 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCTGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5