Detailed information of TA system TA129218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 713919..714600
• Replicon: chromosome
• Accession: NZ_CP041773
• Organism: Pseudomonas aeruginosa strain 519119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FOY95_RS03595
• Protein ID: WP_015503432.1
• Coordinates: 714235..714600 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOY95_RS03590
• Protein ID: WP_016561576.1
• Coordinates: 713919..714242 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY95_RS03550	709878..710321	+	444	WP_014603615.1	SocA family protein	-
FOY95_RS03560	710930..711796	-	867	WP_023081971.1	hypothetical protein	-
FOY95_RS03565	711826..712077	-	252	WP_124136459.1	hypothetical protein	-
FOY95_RS03570	712294..712848	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
FOY95_RS03575	713022..713297	-	276	WP_004353146.1	hypothetical protein	-
FOY95_RS03580	713297..713629	-	333	WP_016561578.1	hypothetical protein	-
FOY95_RS03585	713684..713803	+	120	Protein_684	IS5/IS1182 family transposase	-
FOY95_RS03590	713919..714242	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
FOY95_RS03595	714235..714600	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOY95_RS03600	714864..715106	-	243	WP_043884955.1	hypothetical protein	-
FOY95_RS03605	715313..715585	+	273	WP_003085667.1	hypothetical protein	-
FOY95_RS03610	715604..716029	-	426	WP_003163196.1	VOC family protein	-
FOY95_RS03615	716130..717014	+	885	WP_019725847.1	LysR family transcriptional regulator	-
FOY95_RS03620	716987..717940	-	954	WP_003085661.1	LysR family transcriptional regulator	-
FOY95_RS03625	718161..718595	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	668104..716029	47925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T129218 WP_015503432.1 NZ_CP041773:c714600-714235 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T129218 NZ_CP041773:c714600-714235 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT129218 WP_016561576.1 NZ_CP041773:c714242-713919 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT129218 NZ_CP041773:c714242-713919 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA