Detailed information of TA system TA129211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5587030..5587625
• Replicon: chromosome
• Accession: NZ_CP041771
• Organism: Pseudomonas aeruginosa strain A681

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: FOY61_RS26985
• Protein ID: WP_003113526.1
• Coordinates: 5587347..5587625 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOY61_RS26980
• Protein ID: WP_003099268.1
• Coordinates: 5587030..5587335 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOY61_RS26950	5582706..5582996	-	291	WP_023122804.1	DUF5447 family protein	-
FOY61_RS26955	5583172..5583444	-	273	WP_004352675.1	hypothetical protein	-
FOY61_RS26965	5583891..5584226	+	336	WP_031656002.1	hypothetical protein	-
FOY61_RS26970	5584574..5585494	-	921	WP_160313521.1	retron St85 family RNA-directed DNA polymerase	-
FOY61_RS26975	5585536..5586474	-	939	WP_134455416.1	retron St85 family effector protein	-
FOY61_RS26980	5587030..5587335	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
FOY61_RS26985	5587347..5587625	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FOY61_RS26995	5587954..5590182	+	2229	WP_031633241.1	TonB-dependent receptor	-
FOY61_RS27000	5590252..5590899	-	648	WP_003095021.1	carbonate dehydratase	-
FOY61_RS27005	5590961..5592199	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T129211 WP_003113526.1 NZ_CP041771:c5587625-5587347 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T129211 NZ_CP041771:c5587625-5587347 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT129211 WP_003099268.1 NZ_CP041771:c5587335-5587030 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT129211 NZ_CP041771:c5587335-5587030 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3