Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2779280..2779974 Replicon chromosome
Accession NZ_CP041749
Organism Escherichia coli strain NCCP 15955

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag FOV95_RS13385 Protein ID WP_001263489.1
Coordinates 2779576..2779974 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag FOV95_RS13380 Protein ID WP_000554758.1
Coordinates 2779280..2779573 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOV95_RS13360 2774747..2774956 + 210 WP_061362472.1 hypothetical protein -
FOV95_RS13365 2775054..2777186 + 2133 WP_137528180.1 tape measure protein -
FOV95_RS13370 2777344..2778117 + 774 WP_137528179.1 hypothetical protein -
FOV95_RS13375 2778176..2779228 + 1053 WP_000691315.1 DNA polymerase IV -
FOV95_RS13380 2779280..2779573 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FOV95_RS13385 2779576..2779974 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FOV95_RS13390 2779984..2780436 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
FOV95_RS13395 2780754..2780960 + 207 Protein_2596 RtcB family protein -
FOV95_RS13400 2780956..2781477 + 522 Protein_2597 peptide chain release factor H -
FOV95_RS13405 2781534..2782991 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
FOV95_RS13410 2783252..2783710 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 2784306..2784386 + 81 NuclAT_9 - -
- 2784306..2784386 + 81 NuclAT_9 - -
- 2784306..2784386 + 81 NuclAT_9 - -
- 2784306..2784386 + 81 NuclAT_9 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T129134 WP_001263489.1 NZ_CP041749:2779576-2779974 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T129134 NZ_CP041749:2779576-2779974 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT129134 WP_000554758.1 NZ_CP041749:2779280-2779573 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT129134 NZ_CP041749:2779280-2779573 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References