Detailed information of TA system TA129081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3659184..3659859
• Replicon: chromosome
• Accession: NZ_CP041733
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain ME-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FDC1
• Locus tag: FO617_RS17820
• Protein ID: WP_015571638.1
• Coordinates: 3659184..3659483 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FO617_RS17825
• Protein ID: WP_144427971.1
• Coordinates: 3659494..3659859 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO617_RS17810	3655561..3657753	+	2193	WP_017692801.1	type I secretion system permease/ATPase	-
FO617_RS17815	3657773..3658933	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
FO617_RS17820	3659184..3659483	+	300	WP_015571638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FO617_RS17825	3659494..3659859	+	366	WP_144427971.1	HigA family addiction module antidote protein	Antitoxin
FO617_RS17830	3659886..3661067	-	1182	WP_032669342.1	PLP-dependent aminotransferase family protein	-
FO617_RS17835	3661087..3661974	-	888	WP_015571641.1	LysR family transcriptional regulator	-
FO617_RS17840	3662072..3662674	+	603	WP_017382759.1	short chain dehydrogenase	-
FO617_RS17845	3662671..3663402	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11624.29 Da
• Isoelectric Point: 9.6776

>T129081 WP_015571638.1 NZ_CP041733:3659184-3659483 [Enterobacter hormaechei subsp. steigerwaltii]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T129081 NZ_CP041733:3659184-3659483 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATTCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13696.68 Da
• Isoelectric Point: 8.5370

>AT129081 WP_144427971.1 NZ_CP041733:3659494-3659859 [Enterobacter hormaechei subsp. steigerwaltii]
MTLEQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKSDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT129081 NZ_CP041733:3659494-3659859 [Enterobacter hormaechei subsp. steigerwaltii]
ATGACACTTGAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCGTTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGTCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Toxin

• AlphaFold DB: A0A837FDC1