Detailed information of TA system TA129067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31722..32452
• Replicon: chromosome
• Accession: NZ_CP041733
• Organism: Enterobacter hormaechei subsp. steigerwaltii strain ME-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: FO617_RS00155
• Protein ID: WP_023302825.1
• Coordinates: 31722..32036 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FO617_RS00160
• Protein ID: WP_022646553.1
• Coordinates: 32033..32452 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FO617_RS00130	27388..28473	+	1086	Protein_25	cellulase family glycosylhydrolase	-
FO617_RS00135	28379..29563	-	1185	WP_144427614.1	multidrug efflux MFS transporter EmrD	-
FO617_RS00140	29739..30572	-	834	WP_058687215.1	DMT family transporter	-
FO617_RS00145	30635..31081	-	447	WP_144427615.1	GNAT family N-acetyltransferase	-
FO617_RS00150	31146..31235	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
FO617_RS00155	31722..32036	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
FO617_RS00160	32033..32452	+	420	WP_022646553.1	helix-turn-helix domain-containing protein	Antitoxin
FO617_RS00165	32599..32697	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
FO617_RS00170	32804..34492	+	1689	WP_032672176.1	acetolactate synthase large subunit	-
FO617_RS00175	34496..34783	+	288	WP_015570113.1	acetolactate synthase small subunit	-
FO617_RS00180	34909..35502	+	594	WP_144427616.1	transcriptional regulator UhpA	-
FO617_RS00185	35499..37004	+	1506	WP_033487017.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T129067 WP_023302825.1 NZ_CP041733:31722-32036 [Enterobacter hormaechei subsp. steigerwaltii]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T129067 NZ_CP041733:31722-32036 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAACCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15480.10 Da
• Isoelectric Point: 5.6761

>AT129067 WP_022646553.1 NZ_CP041733:32033-32452 [Enterobacter hormaechei subsp. steigerwaltii]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT129067 NZ_CP041733:32033-32452 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2