Detailed information of TA system TA128986

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43305..43569
• Replicon: plasmid unnamed1
• Accession: NZ_CP041679
• Organism: Escherichia coli strain ESBL 15

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: FOA56_RS25095
• Protein ID: WP_001331364.1
• Coordinates: 43417..43569 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43305..43362 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA56_RS25080	38544..40835	-	2292	WP_001289276.1	hypothetical protein	-
FOA56_RS25085	40828..41898	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
FOA56_RS25090	41917..43125	-	1209	WP_016245570.1	IncI1-type conjugal transfer protein TrbA	-
-	43305..43362	-	58	NuclAT_0	-	Antitoxin
-	43305..43362	-	58	NuclAT_0	-	Antitoxin
-	43305..43362	-	58	NuclAT_0	-	Antitoxin
-	43305..43362	-	58	NuclAT_0	-	Antitoxin
FOA56_RS25095	43417..43569	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
FOA56_RS25100	43641..43892	-	252	WP_032329894.1	hypothetical protein	-
FOA56_RS25615	44551..44727	-	177	WP_001054897.1	hypothetical protein	-
FOA56_RS25105	45119..45328	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FOA56_RS25110	45400..46050	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
FOA56_RS25115	46124..48286	-	2163	WP_149617733.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-1	-	1..88891	88891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T128986 WP_001331364.1 NZ_CP041679:43417-43569 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T128986 NZ_CP041679:43417-43569 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT128986 NZ_CP041679:c43362-43305 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin