Detailed information of TA system TA128985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4800538..4801140
• Replicon: chromosome
• Accession: NZ_CP041678
• Organism: Escherichia coli strain ESBL 15

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FOA56_RS23825
• Protein ID: WP_000897305.1
• Coordinates: 4800829..4801140 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FOA56_RS23820
• Protein ID: WP_000356397.1
• Coordinates: 4800538..4800828 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOA56_RS23795	4796467..4797369	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FOA56_RS23800	4797366..4798001	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FOA56_RS23805	4797998..4798927	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FOA56_RS23810	4799257..4799499	-	243	WP_001086388.1	hypothetical protein	-
FOA56_RS23815	4799718..4799936	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FOA56_RS23820	4800538..4800828	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FOA56_RS23825	4800829..4801140	-	312	WP_000897305.1	hypothetical protein	Toxin
FOA56_RS23830	4801369..4802277	+	909	WP_001162704.1	alpha/beta hydrolase	-
FOA56_RS23835	4802341..4803282	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FOA56_RS23840	4803327..4803764	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FOA56_RS23845	4803761..4804633	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FOA56_RS23850	4804627..4805226	-	600	WP_001295269.1	glucose-1-phosphatase	-
FOA56_RS23855	4805325..4806110	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128985 WP_000897305.1 NZ_CP041678:c4801140-4800829 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128985 NZ_CP041678:c4801140-4800829 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128985 WP_000356397.1 NZ_CP041678:c4800828-4800538 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128985 NZ_CP041678:c4800828-4800538 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7