Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3895308..3895849 Replicon chromosome
Accession NZ_CP041678
Organism Escherichia coli strain ESBL 15

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FOA56_RS19520 Protein ID WP_000615976.1
Coordinates 3895571..3895849 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FOA56_RS19515 Protein ID WP_000729704.1
Coordinates 3895308..3895568 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOA56_RS19490 3891091..3891876 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
FOA56_RS19495 3891848..3893560 + 1713 Protein_3736 flagellar biosynthesis protein FlhA -
FOA56_RS19500 3893665..3893943 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FOA56_RS19505 3893936..3894292 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FOA56_RS19510 3894349..3895122 - 774 WP_000543899.1 C40 family peptidase -
FOA56_RS19515 3895308..3895568 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FOA56_RS19520 3895571..3895849 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FOA56_RS19525 3896005..3896745 + 741 WP_001225679.1 murein L,D-transpeptidase -
FOA56_RS19530 3896716..3897483 - 768 WP_000333380.1 class II glutamine amidotransferase -
FOA56_RS19535 3897689..3898267 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T128980 WP_000615976.1 NZ_CP041678:3895571-3895849 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128980 NZ_CP041678:3895571-3895849 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128980 WP_000729704.1 NZ_CP041678:3895308-3895568 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128980 NZ_CP041678:3895308-3895568 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References