Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1849620..1850122 Replicon chromosome
Accession NZ_CP041678
Organism Escherichia coli strain ESBL 15

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FOA56_RS08995 Protein ID WP_000767829.1
Coordinates 1849868..1850122 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FOA56_RS08990 Protein ID WP_001259255.1
Coordinates 1849620..1849871 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOA56_RS08965 1844798..1845865 - 1068 WP_001567686.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FOA56_RS08970 1845865..1846935 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FOA56_RS08975 1846932..1848236 - 1305 WP_001614652.1 histidinol dehydrogenase -
FOA56_RS08980 1848242..1849141 - 900 WP_000131777.1 ATP phosphoribosyltransferase -
FOA56_RS08985 1849287..1849337 - 51 WP_001364200.1 his operon leader peptide -
FOA56_RS08990 1849620..1849871 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FOA56_RS08995 1849868..1850122 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FOA56_RS09000 1850205..1851029 + 825 WP_000754749.1 SDR family oxidoreductase -
FOA56_RS09005 1851075..1852004 + 930 WP_000803351.1 LysR family transcriptional regulator -
FOA56_RS09010 1852219..1852281 + 63 WP_010723108.1 membrane protein YoeI -
FOA56_RS09015 1852271..1853629 + 1359 WP_053887999.1 putrescine/proton symporter PlaP -
FOA56_RS09020 1853808..1854866 + 1059 WP_000492344.1 transport protein YeeE -
FOA56_RS09025 1854880..1855107 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128968 WP_000767829.1 NZ_CP041678:1849868-1850122 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128968 NZ_CP041678:1849868-1850122 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128968 WP_001259255.1 NZ_CP041678:1849620-1849871 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128968 NZ_CP041678:1849620-1849871 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References