Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 2760753..2761317 Replicon chromosome
Accession NZ_CP041657
Organism Lacticaseibacillus paracasei strain CBA3611

Toxin (Protein)


Gene name yafQ Uniprot ID A0A6G9EML4
Locus tag FGZ69_RS14515 Protein ID WP_014951859.1
Coordinates 2760753..2761055 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag FGZ69_RS14520 Protein ID WP_003570051.1
Coordinates 2761048..2761317 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGZ69_RS14500 2756054..2756983 + 930 WP_003599410.1 IS30-like element ISLpl1 family transposase -
FGZ69_RS14505 2757048..2759225 - 2178 WP_012491514.1 RNA-binding transcriptional accessory protein -
FGZ69_RS14510 2759461..2760153 - 693 WP_012491513.1 hypothetical protein -
FGZ69_RS16375 2760271..2760414 - 144 WP_003574774.1 hypothetical protein -
FGZ69_RS14515 2760753..2761055 - 303 WP_014951859.1 type II toxin-antitoxin system YafQ family toxin Toxin
FGZ69_RS14520 2761048..2761317 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
FGZ69_RS14525 2761691..2762344 - 654 WP_012491511.1 GNAT family N-acetyltransferase -
FGZ69_RS14530 2762389..2763018 - 630 WP_012491510.1 DUF2399 domain-containing protein -
FGZ69_RS14535 2763081..2763407 - 327 WP_012491509.1 YrdB family protein -
FGZ69_RS14540 2763400..2763804 - 405 WP_012491508.1 MerR family transcriptional regulator -
FGZ69_RS14545 2764002..2765228 + 1227 WP_012491507.1 hypothetical protein -
FGZ69_RS14550 2765426..2766313 + 888 WP_024109452.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2756054..2756983 929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T128946 WP_014951859.1 NZ_CP041657:c2761055-2760753 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T128946 NZ_CP041657:c2761055-2760753 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT128946 WP_003570051.1 NZ_CP041657:c2761317-2761048 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT128946 NZ_CP041657:c2761317-2761048 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9EML4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References