Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 58276..58933 | Replicon | plasmid pKP27-MCR1 |
| Accession | NZ_CP041641 | ||
| Organism | Klebsiella pneumoniae strain PIMB15ND2KP27 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | DIJ67_RS27855 | Protein ID | WP_000270043.1 |
| Coordinates | 58583..58933 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | DIJ67_RS27850 | Protein ID | WP_000124640.1 |
| Coordinates | 58276..58578 (-) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DIJ67_RS27800 | 53901..54329 | + | 429 | WP_000591074.1 | hypothetical protein | - |
| DIJ67_RS27805 | 54386..54745 | + | 360 | WP_000422768.1 | hypothetical protein | - |
| DIJ67_RS27810 | 54745..55191 | + | 447 | WP_000919345.1 | Fe3+-siderophore ABC transporter permease | - |
| DIJ67_RS27815 | 55188..55706 | + | 519 | WP_000210756.1 | nitrite reductase | - |
| DIJ67_RS27820 | 55706..55936 | + | 231 | WP_000972663.1 | hypothetical protein | - |
| DIJ67_RS27825 | 55923..56780 | + | 858 | WP_001167032.1 | hypothetical protein | - |
| DIJ67_RS27835 | 57011..57538 | + | 528 | WP_001236377.1 | thermonuclease family protein | - |
| DIJ67_RS27840 | 57596..57868 | + | 273 | WP_001043047.1 | HU family DNA-binding protein | - |
| DIJ67_RS27845 | 57956..58249 | + | 294 | WP_001239997.1 | chromosome segregation protein ParM | - |
| DIJ67_RS27850 | 58276..58578 | - | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| DIJ67_RS27855 | 58583..58933 | - | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| DIJ67_RS27860 | 59096..59644 | + | 549 | WP_001061574.1 | transcriptional regulator | - |
| DIJ67_RS27870 | 59985..60179 | + | 195 | WP_000343597.1 | hypothetical protein | - |
| DIJ67_RS27875 | 60190..60561 | + | 372 | WP_000516916.1 | hypothetical protein | - |
| DIJ67_RS27880 | 60554..61024 | + | 471 | WP_001281821.1 | hypothetical protein | - |
| DIJ67_RS27885 | 61039..61374 | - | 336 | WP_000683476.1 | hypothetical protein | - |
| DIJ67_RS27890 | 61471..61959 | + | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| DIJ67_RS27895 | 61962..62459 | + | 498 | WP_000062185.1 | hypothetical protein | - |
| DIJ67_RS27905 | 62674..62880 | - | 207 | Protein_81 | DDE-type integrase/transposase/recombinase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mcr-1.1 | - | 1..144138 | 144138 | |
| - | flank | IS/Tn | - | - | 62674..62868 | 194 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T128918 WP_000270043.1 NZ_CP041641:c58933-58583 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T128918 NZ_CP041641:c58933-58583 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT128918 WP_000124640.1 NZ_CP041641:c58578-58276 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT128918 NZ_CP041641:c58578-58276 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|