Detailed information of TA system TA128899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 354975..355615
• Replicon: chromosome
• Accession: NZ_CP041639
• Organism: Klebsiella pneumoniae strain PIMB15ND2KP27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: DIJ67_RS01655
• Protein ID: WP_016529833.1
• Coordinates: 354975..355322 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DIJ67_RS01660
• Protein ID: WP_040181955.1
• Coordinates: 355322..355615 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DIJ67_RS01645	350901..352334	+	1434	WP_002920564.1	glycogen synthase GlgA	-
DIJ67_RS01650	352352..354799	+	2448	WP_002920561.1	glycogen phosphorylase	-
DIJ67_RS01655	354975..355322	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DIJ67_RS01660	355322..355615	+	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
DIJ67_RS01665	355685..357193	-	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
DIJ67_RS01670	357398..357727	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
DIJ67_RS01675	357778..358608	+	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
DIJ67_RS01680	358658..359416	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T128899 WP_016529833.1 NZ_CP041639:354975-355322 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T128899 NZ_CP041639:354975-355322 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT128899 WP_040181955.1 NZ_CP041639:355322-355615 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT128899 NZ_CP041639:355322-355615 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Structures

Toxin

• AlphaFold DB: W9BBY1