Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4132763..4133304 Replicon chromosome
Accession NZ_CP041628
Organism Escherichia coli strain PE15

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag FNZ59_RS19915 Protein ID WP_000615979.1
Coordinates 4132763..4133041 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FNZ59_RS19920 Protein ID WP_000729704.1
Coordinates 4133044..4133304 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNZ59_RS19900 4130345..4130923 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FNZ59_RS19905 4131129..4131896 + 768 WP_000333379.1 class II glutamine amidotransferase -
FNZ59_RS19910 4131867..4132607 - 741 WP_001225679.1 murein L,D-transpeptidase -
FNZ59_RS19915 4132763..4133041 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNZ59_RS19920 4133044..4133304 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNZ59_RS19925 4133490..4134263 + 774 WP_001353765.1 C40 family peptidase -
FNZ59_RS19930 4134439..4134936 + 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
FNZ59_RS19935 4135152..4136864 - 1713 Protein_3884 flagellar biosynthesis protein FlhA -
FNZ59_RS19940 4136836..4137621 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4124437..4158449 34012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T128887 WP_000615979.1 NZ_CP041628:c4133041-4132763 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128887 NZ_CP041628:c4133041-4132763 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128887 WP_000729704.1 NZ_CP041628:c4133304-4133044 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128887 NZ_CP041628:c4133304-4133044 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References