Detailed information of TA system TA128884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2451124..2451923
• Replicon: chromosome
• Accession: NZ_CP041628
• Organism: Escherichia coli strain PE15

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: FNZ59_RS12030
• Protein ID: WP_000347273.1
• Coordinates: 2451459..2451923 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: FNZ59_RS12025
• Protein ID: WP_021547164.1
• Coordinates: 2451124..2451459 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNZ59_RS12010	2446909..2447679	-	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-
FNZ59_RS12015	2447695..2449029	-	1335	WP_077492475.1	galactarate/glucarate/glycerate transporter GarP	-
FNZ59_RS12020	2449404..2450975	+	1572	WP_001753084.1	galactarate dehydratase	-
FNZ59_RS12025	2451124..2451459	+	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FNZ59_RS12030	2451459..2451923	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FNZ59_RS12035	2451978..2452787	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FNZ59_RS12040	2453036..2454316	+	1281	WP_042096485.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FNZ59_RS12045	2454339..2454812	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FNZ59_RS12050	2454823..2455602	+	780	WP_077492473.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
FNZ59_RS12055	2455592..2456471	+	880	Protein_2362	PTS mannose/fructose/sorbose transporter family subunit IID	-
FNZ59_RS12060	2456489..2456923	+	435	WP_000948814.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T128884 WP_000347273.1 NZ_CP041628:2451459-2451923 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T128884 NZ_CP041628:2451459-2451923 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGGCATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT128884 WP_021547164.1 NZ_CP041628:2451124-2451459 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT128884 NZ_CP041628:2451124-2451459 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39