Detailed information of TA system TA128865

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 452607..452978
• Replicon: chromosome
• Accession: NZ_CP041628
• Organism: Escherichia coli strain PE15

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: FNZ59_RS02240
• Protein ID: WP_001317028.1
• Coordinates: 452784..452978 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	452607..452785 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNZ59_RS02215	448562..449935	+	1374	WP_077492676.1	ATP-dependent RNA helicase DbpA	-
FNZ59_RS02220	450064..450999	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
FNZ59_RS02225	451051..452286	-	1236	WP_000040852.1	site-specific integrase	-
FNZ59_RS02230	452288..452503	-	216	WP_000079604.1	excisionase XisR	-
FNZ59_RS02235	452582..452791	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	452607..452785	+	179	NuclAT_0	-	Antitoxin
-	452607..452785	+	179	NuclAT_0	-	Antitoxin
-	452607..452785	+	179	NuclAT_0	-	Antitoxin
-	452607..452785	+	179	NuclAT_0	-	Antitoxin
FNZ59_RS02240	452784..452978	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FNZ59_RS02245	453035..453844	-	810	WP_000166319.1	recombination protein RecT	-
FNZ59_RS02250	453837..455717	-	1881	Protein_439	exodeoxyribonuclease VIII	-
FNZ59_RS02255	455717..456040	+	324	Protein_440	lysis protein	-
FNZ59_RS02260	456178..457635	+	1458	WP_001097895.1	Trk system potassium transporter TrkG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	451051..458423	7372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T128865 WP_001317028.1 NZ_CP041628:c452978-452784 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T128865 NZ_CP041628:c452978-452784 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT128865 NZ_CP041628:452607-452785 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin