Detailed information of TA system TA128843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256449..257143
• Replicon: chromosome
• Accession: NZ_CP041627
• Organism: Escherichia coli strain ETEC6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FNW97_RS01185
• Protein ID: WP_001263493.1
• Coordinates: 256745..257143 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FNW97_RS01180
• Protein ID: WP_000554757.1
• Coordinates: 256449..256742 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNW97_RS01165	252802..254541	-	1740	WP_047603826.1	flagellar biosynthesis protein FlhA	-
FNW97_RS01170	254486..255271	+	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
FNW97_RS01175	255342..256397	+	1056	WP_001226164.1	DNA polymerase IV	-
FNW97_RS01180	256449..256742	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNW97_RS01185	256745..257143	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNW97_RS01190	257153..257605	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FNW97_RS01195	257851..258054	+	204	Protein_231	RNA ligase RtcB family protein	-
FNW97_RS01200	258053..258574	+	522	Protein_232	peptide chain release factor H	-
FNW97_RS01205	258631..260088	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FNW97_RS01210	260349..260807	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FNW97_RS01215	260899..262143	+	1245	WP_000189541.1	esterase FrsA	-
-	261403..261483	+	81	NuclAT_12	-	-
-	261403..261483	+	81	NuclAT_12	-	-
-	261403..261483	+	81	NuclAT_12	-	-
-	261403..261483	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T128843 WP_001263493.1 NZ_CP041627:256745-257143 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128843 NZ_CP041627:256745-257143 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT128843 WP_000554757.1 NZ_CP041627:256449-256742 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128843 NZ_CP041627:256449-256742 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6