Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 249064..249605 Replicon chromosome
Accession NZ_CP041627
Organism Escherichia coli strain ETEC6

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag FNW97_RS01140 Protein ID WP_000615979.1
Coordinates 249064..249342 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FNW97_RS01145 Protein ID WP_000729704.1
Coordinates 249345..249605 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW97_RS01125 246646..247224 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FNW97_RS01130 247430..248197 + 768 WP_000333379.1 class II glutamine amidotransferase -
FNW97_RS01135 248168..248908 - 741 WP_001225679.1 murein L,D-transpeptidase -
FNW97_RS01140 249064..249342 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FNW97_RS01145 249345..249605 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FNW97_RS01150 249791..250564 + 774 WP_001353765.1 C40 family peptidase -
FNW97_RS01155 250740..251264 + 525 WP_149796506.1 REP-associated tyrosine transposase RayT -
FNW97_RS01160 251375..252487 - 1113 WP_001300563.1 IS4-like element IS421 family transposase -
FNW97_RS01165 252802..254541 - 1740 WP_047603826.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 234381..252487 18106


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T128842 WP_000615979.1 NZ_CP041627:c249342-249064 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T128842 NZ_CP041627:c249342-249064 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT128842 WP_000729704.1 NZ_CP041627:c249605-249345 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT128842 NZ_CP041627:c249605-249345 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References