Detailed information of TA system TA128804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 27538..28232
• Replicon: chromosome
• Accession: NZ_CP041623
• Organism: Escherichia coli O157:H7 strain ATCC 43888

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: FNZ21_RS00195
• Protein ID: WP_001263495.1
• Coordinates: 27538..27936 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FNZ21_RS00200
• Protein ID: WP_000554757.1
• Coordinates: 27939..28232 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	23125..23205	-	81	NuclAT_12	-	-
-	23125..23205	-	81	NuclAT_12	-	-
-	23125..23205	-	81	NuclAT_12	-	-
-	23125..23205	-	81	NuclAT_12	-	-
FNZ21_RS00170	23801..24259	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FNZ21_RS00175	24520..25977	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
FNZ21_RS00180	26034..26555	-	522	Protein_33	peptide chain release factor H	-
FNZ21_RS00185	26554..26757	-	204	Protein_34	RNA ligase RtcB family protein	-
FNZ21_RS00190	27076..27528	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
FNZ21_RS00195	27538..27936	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNZ21_RS00200	27939..28232	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNZ21_RS00205	28284..29339	-	1056	WP_001226188.1	DNA polymerase IV	-
FNZ21_RS00210	29410..30195	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
FNZ21_RS00215	30167..31879	+	1713	Protein_40	flagellar biosynthesis protein FlhA	-
FNZ21_RS00220	32024..32521	-	498	Protein_41	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T128804 WP_001263495.1 NZ_CP041623:c27936-27538 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128804 NZ_CP041623:c27936-27538 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT128804 WP_000554757.1 NZ_CP041623:c28232-27939 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128804 NZ_CP041623:c28232-27939 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6