Detailed information of TA system TA128800

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19136..19400
• Replicon: plasmid pC32_2
• Accession: NZ_CP041621
• Organism: Shigella flexneri strain C32

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: FNL95_RS25090
• Protein ID: WP_001331364.1
• Coordinates: 19136..19288 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	19343..19400 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNL95_RS25070	14413..16581	+	2169	WP_000698357.1	DotA/TraY family protein	-
FNL95_RS25075	16655..17305	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
FNL95_RS25080	17377..17586	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FNL95_RS25745	17978..18154	+	177	WP_001054898.1	hypothetical protein	-
FNL95_RS25085	18813..19064	+	252	WP_001291964.1	hypothetical protein	-
FNL95_RS25090	19136..19288	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	19343..19400	+	58	NuclAT_0	-	Antitoxin
-	19343..19400	+	58	NuclAT_0	-	Antitoxin
-	19343..19400	+	58	NuclAT_0	-	Antitoxin
-	19343..19400	+	58	NuclAT_0	-	Antitoxin
-	19641..19696	+	56	NuclAT_1	-	-
-	19641..19696	+	56	NuclAT_1	-	-
-	19641..19696	+	56	NuclAT_1	-	-
-	19641..19696	+	56	NuclAT_1	-	-
FNL95_RS25095	19857..20843	+	987	WP_001257838.1	hypothetical protein	-
FNL95_RS25100	21061..22269	+	1209	WP_154832363.1	IncI1-type conjugal transfer protein TrbA	-
FNL95_RS25105	22288..23358	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88076	88076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T128800 WP_001331364.1 NZ_CP041621:c19288-19136 [Shigella flexneri]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T128800 NZ_CP041621:c19288-19136 [Shigella flexneri]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT128800 NZ_CP041621:19343-19400 [Shigella flexneri]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin