Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4885755..4886257 Replicon chromosome
Accession NZ_CP041620
Organism Shigella flexneri strain C32

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag FNL95_RS24670 Protein ID WP_000767822.1
Coordinates 4886003..4886257 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FNL95_RS24665 Protein ID WP_001259255.1
Coordinates 4885755..4886006 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNL95_RS24640 4880933..4882000 - 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FNL95_RS24645 4882000..4883070 - 1071 WP_001538918.1 histidinol-phosphate transaminase -
FNL95_RS24650 4883067..4884371 - 1305 WP_001445483.1 histidinol dehydrogenase -
FNL95_RS24655 4884377..4885276 - 900 WP_000131779.1 ATP phosphoribosyltransferase -
FNL95_RS24660 4885422..4885472 - 51 WP_001364200.1 his operon leader peptide -
FNL95_RS24665 4885755..4886006 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FNL95_RS24670 4886003..4886257 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FNL95_RS24675 4886340..4887164 + 825 WP_000754737.1 SDR family oxidoreductase -
FNL95_RS24680 4887210..4888139 + 930 WP_000803366.1 LysR family transcriptional regulator -
FNL95_RS24685 4888354..4888416 + 63 WP_010723108.1 membrane protein YoeI -
FNL95_RS24690 4888406..4889764 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T128799 WP_000767822.1 NZ_CP041620:4886003-4886257 [Shigella flexneri]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T128799 NZ_CP041620:4886003-4886257 [Shigella flexneri]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT128799 WP_001259255.1 NZ_CP041620:4885755-4886006 [Shigella flexneri]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT128799 NZ_CP041620:4885755-4886006 [Shigella flexneri]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References