Detailed information of TA system TA128793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2796180..2796782
• Replicon: chromosome
• Accession: NZ_CP041620
• Organism: Shigella flexneri strain C32

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: FNL95_RS14780
• Protein ID: WP_000897302.1
• Coordinates: 2796471..2796782 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FNL95_RS14775
• Protein ID: WP_000356397.1
• Coordinates: 2796180..2796470 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNL95_RS14740	2792253..2793155	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FNL95_RS14745	2793152..2793787	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FNL95_RS14750	2793784..2794713	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FNL95_RS14755	2794929..2795147	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
FNL95_RS14765	2795543..2795821	-	279	WP_001296612.1	hypothetical protein	-
FNL95_RS14775	2796180..2796470	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FNL95_RS14780	2796471..2796782	-	312	WP_000897302.1	hypothetical protein	Toxin
FNL95_RS14785	2797011..2797919	+	909	WP_001774092.1	alpha/beta hydrolase	-
FNL95_RS14790	2797983..2798924	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FNL95_RS14795	2798969..2799406	-	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FNL95_RS14800	2799403..2800275	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FNL95_RS14805	2800269..2800868	-	600	WP_001314324.1	glucose-1-phosphatase	-
FNL95_RS14810	2800967..2801752	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T128793 WP_000897302.1 NZ_CP041620:c2796782-2796471 [Shigella flexneri]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128793 NZ_CP041620:c2796782-2796471 [Shigella flexneri]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128793 WP_000356397.1 NZ_CP041620:c2796470-2796180 [Shigella flexneri]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128793 NZ_CP041620:c2796470-2796180 [Shigella flexneri]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416