Detailed information of TA system TA128782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1765107..1765801
• Replicon: chromosome
• Accession: NZ_CP041620
• Organism: Shigella flexneri strain C32

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: FNL95_RS09715
• Protein ID: WP_001263500.1
• Coordinates: 1765107..1765505 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: FNL95_RS09720
• Protein ID: WP_000554758.1
• Coordinates: 1765508..1765801 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FNL95_RS09690	1760472..1760930	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
FNL95_RS09695	1761191..1762648	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
FNL95_RS09700	1762705..1763319	-	615	WP_000602124.1	peptide chain release factor H	-
FNL95_RS09705	1763316..1764455	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
FNL95_RS09710	1764645..1765097	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
FNL95_RS09715	1765107..1765505	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FNL95_RS09720	1765508..1765801	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FNL95_RS09725	1765853..1766908	-	1056	WP_001226168.1	DNA polymerase IV	-
FNL95_RS09730	1766979..1767902	-	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
FNL95_RS09735	1767905..1768768	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
FNL95_RS09740	1768781..1769497	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
FNL95_RS09745	1769517..1769984	-	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T128782 WP_001263500.1 NZ_CP041620:c1765505-1765107 [Shigella flexneri]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T128782 NZ_CP041620:c1765505-1765107 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT128782 WP_000554758.1 NZ_CP041620:c1765801-1765508 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT128782 NZ_CP041620:c1765801-1765508 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3